The Effects of Giving Reused Cooking Oil 1,5 mL/day for 14 Days on Male Rattus norvegicus Liver Histopathology
DOI:
https://doi.org/10.53089/medula.v14i5.1088Keywords:
Liver histopathology, Reused cooking oil, Rats, Toxicity, liver histopatology, reused cooking oil, rats toxicityAbstract
Using cooking oil multiple times can caused bad effect on health one of them is liver damage. Free radical content in reused cooking oil trigger oxidative stress then disturb the cellular respons in liver. This study aimed to determine the effect of giving reused cooking oil for 14 days on rats (Rattus norvegicus) liver histopathology. This study was a true experimental using 10 rats (Rattus norvegicus) and were devided by 2 groups, which are normal group (KN) and treatment group (KP) that was given 1,5 mL reused cooking oil per day. After 14 days of treatment, rats were terminated and the liver were taken. To observe rats liver histopathology, cloudy swelling scoring were used on 5 field of view. Score 0: there was no cloudy swelling and necrosis found, score 1: there was 1-20% cloudy swelling and necrosis found, score 2: there was 21-50% cloudy swelling and necrosis found, score 3: there was 51-75% cloudy swelling and necrosis found, and score 4: there was more than 75% cloudy swelling and necrosis found.It was found a minimal cloudy swelling on KN with average liver damage score was 0,04. In group KP it were found cloudy swelling degeneration on all over the liver with average liver damage score was 4. Mann-Whitney test showed a significant difference between groups. Giving reused cooking oil 1,5 ml/day for 14 days caused damage on male rats liver histopathology.
References
Skeaff MC, Mann J. Lipids. Dalam: Mann J. Truswell S. penyunting. Essentials of human nutrition. Edisi ke-5. Oxford; 2017.
Hafizah D. Hakim DB, Nurmalina R. Analysing Food Consumption in Indonesia. IJPSAT. 2021; 5(2): 340-347.
PAPSI. Minyak Goreng Sawit Dalam Perubahan Konsumsi Minyak Goreng di Indonesia. Palm oil Journal. 2021; 1(25): 434-483.
Tanaem MG. Ernah. Perilaku Konsumsi Minyak Goreng Sawit Selama Masa Pandemi COVID-19 di Kota Bandung Jawa Barat. Agritech. 2021;23(1): 10-16.
Gibson M. Newsham P. Lipids, Oils, Fats, and Extracts. Elsevier Inc. 2018.
Affrizal A. Hierdawati T. Dani R. 2022. Cooking Oil Scarcity Phenomenon In Indonesia In 2022. International Journal Of Artificial Intelegence Research. 2022;6(1): 2579–2798.
Rahayu RN. Kenaikan Harga Minyak Goreng Kelapa Sawit di Indonesia Sebuah Analisis Berita Kompas Online. Intelektiva. 2022;3(8): 26-37.
Muntazah A. Emeilia RI. Novalia. Perubahan Perilaku Konsumen pada Fenomena Kelangkaan Minyak Goreng di Pasaran. Jurnal Pariwara. 2022;2(2): 74– 80.
Warsiki E. Iskandar A. Hidayati M. Degradation quality of reused palm cooking oil during storage: case study in fried shallot industry. IOP Conference Series: Earth and Environmental Science. 2020;60(1): 1-10.
Taufik M. Seftiono H. Karakteristik Fisik dan Kimia Minyak Goreng Sawit Hasil Penggorengan Dengan Metode Deep-Fat Frying. Jurnal Teknologi. 2018;10(2): 123-130.
Jaarin K. Masbah N.Kamisah Y. Heated Oil and Its Effect on Health. In Food Quality: Balancing Health and Disease. Elsevier Inc. 2018.
Sari MN. Rohmawati DL. The Relationship Between Behavior of Using Repeated Cooking Oils and Recurrence of Hypertension. Journal of Vocational Nursing. 2021;2(1): 62-61.
Sree SR. Suneetha WJ. Fats and Oils : Effects of Processing and Its Oxidation, 2022;11(42), 226–230.
Mustofa S. Bahagia W. Kurniawaty E. dkk. The Effect of Mangrove (Rhizospora apiculate) Bark Extract Ethanol On Histopathology Pancreas Of Male White Rats Sprague Dawley Strain Exposed To Cigarette Smoke. Acta Biochimica Indonesiana.2018;1(1): 7-13.
Kurniawaty E. dkk. Ethanol extract of Bruguirea gymnorrhiza mangrove leaves and propolis activity on macroscopic healing of cuts in vivo. Acta Biochimica Indonesiana. 2022;5(1): 1-5.
Allameh A.Niayesh R. Aliarab A. Sebastiani G. Pantopoulos, K. Oxidative Stress in Liver Pathophysiology and Disease. Antioxidants. 2023;12(9): 1-23.
Aisyah S. Budiman H. Aliza D. Salim MN. Balqis U. Armansyah T. Efek Pemberian Minyak Jelantah Terhadap Gambaran Histopatologi Hati Tikus Putih (Rattus norvegicus) The effect of Administrating Waste Cooking Oil to Histopathology of Rat ( Rattus norvegicus ) liver. Jurnal Medika Veterinaria. 2015;9(1), 26–9.
Mustofa S. Anisya V. Efek Hepatoprotektif Ekstrak Etanol Rhizophora Apiculata Pada Tikus Yang Dipaparkan Asap Rokok. JK Unila. 2020;4(1): 12- 17.
Mustikasari I. Saktini F. Gumay A. R. Pengaruh Frekuensi Penggorengan Minyak Jelantah Terhadap Hepar Tikus Wistar (Rattus Novergicus). Diponegoro Medical Journal. 2019; 8(3), 1000–1010.
Bellanti F. Villani R. Facciorusso A. Vendemiale G. Serviddio G. Lipid oxidation products in the pathogenesis of non-alcoholic steatohepatitis. Free Radical Biology and Medicine. 2017. 173–185.
Muhartono P. Putri NT. Sari TN. Oktafany. Minyak Jelantah Menyebabkan Kerusakan pada Arteri Koronaria, Miokardium, dan Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Sprague dawley. Jurnal Kedokteran Universitas Lampung, 2018;2(2): 129–135.
Mustofa S. Ciptaningrum I. Zuya CS. Subacute Toxicity Test Of Rhizophora apiculata Bark Extract On Liver And Pancreas Histopathology Of Rats. Acta Biochimica Indonesiana, 2020;3(2): 89-97.
Mustofa, S. Fahmi, ZY. Efek Protektive Kardivaskular Ekstrak Rhizophora Apiculata Berbagai Pelarut pada Tikus Yang Dipaparkan Asap Rokok. JK Unila. 2021;5(1): 7-15
Mustofa S. Ananta GAPYV. Oral Administration of Moringa Leaf Ethanol Extract (Moringa Oleifera) for 14 Days Protects The Liver of Male White Rats (Rattus norvegicus) from Acute Damage Caused by High Doses of Paracetamol. Sriwijaya Journal of Medicine. 2022;5(3): 142-149.
Habarakada A. Perumpuli PABN. Thathsaranee WTV. Wanninaika IP. Physical, chemical, and nutritional quality parameters of three different types of oil: Determination of their reusability in deep frying. Food Research. 2021;5(5): 226–235.
Elbialy BES. Saleh NT. Elkhair RMA. Potential Hazard of Feeding Albino Rats On Diet Containing Repeatedly Boiled Cooking Oil: Clinicopathological and Toxicology Studies. International Journal of Advance Research. 2015;3(3): 134-147.
Leong XF. Ng CY. Jaarin K., Mustafa M. Effects of repeated heating of cooking oils on antioxidant content and endothelial function. Austin Journal of Pharmacology and Therapeutics. 2015;3(2): 1-7
Rajendran P. Alzahrani AM. Rengarajan T. Veeraraghavan VP. Krishna S. Consumption of reused vegetable oil intensifies BRCA1 mutations. Critical Reviews in Food Science and Nutrition. 2022;62(5):1222–1229.
Brunt EM. Gouw ASH. Hubscher SG. Tiniakos DG. Bedossa P. Burt AD. Pathology of the liver sinusoids. Histopathology. 2014;64(7): 907–920.
Sheka AC. Adeyi O. Thompson J. Hameed B. Crawford PA. Ikramuddin S. Nonalcoholic Steatohepatitis: A Review. JAMA - Journal of the American Medical Association. 2020;323(12): 1175–1183.
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