Pengaruh Pemberian Ekstrak Etanol Rimpang Lengkuas (Alpinia galanga) terhadap Gambaran Histopatologi Testis Mencit (Mus musculus L.) jantan yang Diinduksi Monosodium Glutamat

Annisa Shafira Pramono; Muhartono; Rizki Hanriko; Eca Cania

doi:10.53089/medula.v10i1.43

Authors

Annisa Shafira Pramono
Muhartono
Rizki Hanriko
Eca Cania

DOI:

https://doi.org/10.53089/medula.v10i1.43

Keywords:

Galangal extract, monosodium glutamate, testicular histopathology.

Abstract

Monosodium Glutamate (MSG) may form free radicals that cause damages to the organs of the body, one of which is the male reproductive organs, i.e. testes. Galangal rhizome is one of the natural ingredients with antioxidant content that can neutralize free radicals. The purpose of this research was to determine the effect of ethanol extract of galangal rhizome on testicular histopathology of MSG-induced male mice. The design of this research was experimental research with 5 treatment groups, each group consisted of 5 mice (Mus musculus L.) DDY strain. Group K (-) was not treated; k (+) was provided with 4 mg/ grBB MSG; P1 was provided with 4 mg/ grBB MSG + 14 mg/ 20 grBB ethanol extract of galangal rhizome; P2 was provided with 4 mg/ grBB MSG + 28 mg/ 20 grBB ethanol extract of galangal rhizome; P3 was provided with 4 mg/ grBB MSG + 56 mg/ 20 grBB ethanol extract of galangal rhizome. Assessment used Johnsen score in group K(-) was 48,4, K(+) was 47,4, P1 was 47,4, P2 was 47,4, and P3 was 48. Kruskal-Wallis test result was p=0,085 (p>0,05). The conclusion was that ethanol extract of galangal rhizome did not effect significantly (p>0,05) on the testicular histopathology of monosodium glutamate-induced mice.

References

Rangan C, Barceloux DG. Food additives and sensitivities. Dis Mon. 2009;55(5):292-311.

Sukawan UY. Efek toksik monosodium glutamat (MSG) pada binatang percobaan. Jurnal Sutisning. 2008;3(2):306-14.

Muharani, Eriska. Pengaruh pemberian MSG (Monosodium Glutamate) pada tikus sprague-dawley betina usiareproduktif selama 2 minggu terhadap kadar enzim penanda kerusakan sel hati (AST/ALT) [skripsi]. Jakarta: Universitas Negeri Syarif Hidayatullah;2016.

Iamsaard S, Sukhorum W, Samrid R, Yimdee J, Kanla, Pipathong. The sensitivity of male rat reproductive organs to monosodium glutamate. Khon Kaen: Khon Kaen University. 2014;43(1):3-9.

Hadjizadeh MR, Tavakol AJ, Barati M. The inhibitory effects of ethanolic extract alpinia galangal on colonic cancer cells (HT-29) and L929 Cells in Vitro. Iran J Cancer Prev.2009;3(69):572–82.

Singh B, Gupta V, Bansal P, Singh R, Kumar D. Pharmacological potential of plant used as aphrodisiacs. Int. J. Pharm. Sci.Rev.Res. 2010;5(1):104-13.

Anindita K. Pengaruh pemberian vitamin c terhadap berat testis, jumlah sel leydig, dan diameter tubulus seminiferus mencit jantan dewasa (Mus musculusL.) yang diinduksi monosodium glutamat (MSG) [skripsi]. Lampung: Universitas Lampung;2012.

Nosseir NS, Ali MHM, Ebaid HM. A histological and morphometric study of monosodium glutamate toxic effect on testicular structure and potentiality of recovery in adult albino rats. Research Journal of Biology. 2012;2:66-78.

Igwebuike UM, Ochiogu IS, Ihedinihu BC, Ikokide JE, Idika IK. The effects of oral administration of monosodium glutamate (MSG) on the testicular morphology and cauda epididymal sperm reserves of young and adult male rats. Veterinarski Arhiv. 2011;8(1):525-34.

Aiache JM, Devissaguet P, Guyot-herman AM. Farmasetika 2. Edisi ke-2. Surabaya: Airlangga University Press; 1993.

Turner PV, Brabb T, Cynthia P, Vasbinder MA. Administration of substances to laboratory animals: routes of administration and factors to consider. J Am Assoc Lab Anim Sci.2011;50(5):600-13.

Ganiswarna S. Farmakologi dan terapi. Edisi ke-4. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia; 1995.

Anas Y, Faozi I, Suharjono. Potensi fraksi n-heksan ekstrak etanol rimpang lengkuas [alpinia galanga (L.) Swartz.] dalam meningkatkan kualitas sperma dan

spermatogenesis. Semarang: Universitas Wahin Hasyim;2015.

Mazaheri M, Shahdadi, Vahid, Boron, Ashraf. Molecullar and biochemical effect of alcohlic extract of alpinia galanga on rat spermatogenesis process. Iran J Reprod Med. 2014;12(11):765-70.