The Effect Of Garlic Extract (Allium Sativum) on Glucose Level and Pancreas Histopathological Images in White Rats (Rattus Norvegicus) Sprague-Dawley Strain Induced by Streptozotocin
DOI:
https://doi.org/10.53089/medula.v14i6.1138Keywords:
diabetes, garlic, glucose, pancreasAbstract
Diabetes mellitus (DM) is one of the highest causes of death in the world whose prevalence increases every year, DM is characterized by elevated blood glucose levels and damage of the pancreas. Streptozotocin is a drug used to induce diabetes mellitus in experimental animals, single high dose administration can cause type 1 DM. Garlic has various component that have the potential as antidiabetic, anti-inflammatory and antioxidant. This study aim to determine the effect of garlic extract (Allium sativum) on blood glucose levels and pancreas histopathological images in white rats (Rattus norvegicus) Sprague-Dawley strain induced by streptozotocin. This research is an experimental study with a post test only control group design. This study used 24 rats and divided into 4 group, K- (normal group), K+ (STZ 60 mg/kgBB), P1 (STZ + garlic extract 500 mg/kgbb), P2 (STZ + garlic extract 750mg/kgBB). Blood glucose levels were assessed with a glucometer and the histopathological images of the pancreas was assessed by counting the number and size of the langerhans islet. The mean blood glucose levels for each group were K- = 73.3mg/dL, K+ = 400 mg/dL, P1=203.8 mg/dL, and P2=132.3 mg/dL. One Way ANOVA test obtained p value = 0.000. The mean number of langerhans islet in 10 fields of view obtained K- = 26.1; K+ = 4.6; P1= 7.8; P2=15.1. One Way ANOVA test obtained p = 0.000. The mean area of the langerhans islet in each group obtained K- = 19092.4 µm2; K+ = 4759.3 µm2; P1=8561,4 µm2; P2=9621.8 µm2. Kruskal-Wallis test obtained p = 0.000 (p <0.05). There is an effect of garlic extract (Allium sativum) on blood glucose levels and pancreas histopathological images in white rats (Rattus norvegicus) Sprague-Dawley strain induced by streptozotocin.
References
WHO. Noncommunicable diseases: Mortality. World Health Organization; 2019.
Kementrian kesehatan republik indonesia. Tetap Produktif, Cegah Dan Atasi Diabetes Melitus; 2020.
Dinas Kesehatan Provinsi Lampung. Profil Kesehatan Provinsi Lampung 2019; 2019.
IDF. What Is Diabetes. International Diabetes Federation; 2020.
Cerf ME. Beta cell dysfunction and insulin resistance. Journal Frontiers in Endocrinology. 2013;4(37):1–12.
Rojas J, Bermudez V, Palmar J, Martínez MS, Olivar LC, Nava M et al. Pancreatic beta cell death: Novel potential mechanisms in diabetes therapy. Journal of Diabetes Research. 2018;2018(1):1-19.
Goud BJ, Dwarakanath V, Swamy B. Streptozotocin - A Diabetogenic Agent in Animal Models. Journal Of Pharmacy & Pharmatical Research. 2015;3(1):253-69.
Wu J, Yan LJ. Streptozotocin-induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic β cell glucotoxicity. Journal Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 2015;8(1):181–8.
Moulia MN, Syarief R, Iriani ES, Kusumaningrum HD, Suyatma NE.Antimikroba Ekstrak Bawang Putih. Jurnal Pangan. 2018;27(1):55–66.
Herman M, Syafiftri NA. Inventarisasi tanaman obat tradisional untuk penderita diabetes melitus dan hipertensi di desa minanga kecamatan bambang kabupaten mamasa. Jurnal Farmasi Sandi Karsa. 2017;5(1):1689–99.
Batiha GES, Beshbishy AM, Wasef LG, Elewa YHA, Al-Sagan AA, El-Hack MEA et al. Chemical constituents and pharmacological activities of garlic (Allium sativum L.): A review. Journal Nutrients. 2020;12(3):1–21.
Shang A, Cao SY, Xu XY, Gan RY, Tang GY, Corke H et al. Bioactive compounds and biological functions of garlic (Allium sativum L.). Journal Foods. 2019;8(7):1–31.
Nubatonis DC, Ndaong NA, Selan YN. Pengaruh pemberian ekstrak etanol daun sambiloto (Andrographis paniculata nees) terhadap histopatologi pankreas mencit (Mus musculus) diabetes melitus (dm) tipe 1. Jurnal Kajian Veteriner. 2015;3(1):31-40
Aster KA. Buku Ajar Patologi Robbins Edisi 9. Singapura : Elsevier; 2015.
Seiron P, Wiberg A, Kuric E, Krogvold L, Jahnsen FL, Skog O et al. Characterisation of endocrine pancreas in type 1 diabetes : islet size is maintained but islet number is markedly reduced. Journal of pathology. 2019;5(1):248-55.
Rohmah, MK. Studi in silico potensi senyawa alliin bawang putih (allium sativum) sebagai inhibitor dpp-4 pada diabetes melitus. Jurnal Ilmiah Medicamento. 2018;4(1)-13-7.
Yun HM, Ban JO, Park KR, Lee CK, Jeong HS, Han SB, et al. Potential therapeutic effects of functionally active compounds isolated from garlic. Journal Pharmacology & Therapeutics. 2013;142(1):183-95.
Zhai B, Zhang C, Sheng Y, Zhao C, He X, Xu W. Hypoglycemic and hypolipidemic efect of S-allyl-cysteine sulfoxide (alliin) in DIO mice. Journal Scientific Reports. 2018;8(1):1-7.
Rofiati H, Suwandito, Wahjudi RM. Jus bawang putih dapat mempertahankan kadar glukosa darah puasa normal tikus wistar. Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga. 2017;9(1):26-30.
Cahya BP, Mambo C, Wowor MP. Uji efek ekstrak umbi bawang putih (Allium sativum l.) terhadap kadar glukosa darah tikus wistar (Rattus norvegicus) yang diinduksi aloksan. Jurnal E-Biomedik. 2015;3(1):1-6.
Shakya VK, Saxena RC, Shakya A. Effect of ethanolic extract of Allium sativum bulbs on Streptozotocin induced diabetic rats. Journal Chem. Pharn. Res. 2010;2(6):171-5.
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