The Effect of Vitamin C in Pyramidal Cell Count of CA1 Layer of The Hippocampus on Male Albino Rat (Rattus norvegicus) Sprague Dawley Strain Induced by Monosodium Glutamate (MSG)

Authors

  • Shinta Melia Desiana Lampung University
  • Susianti Susianti Universitas Lampung
  • Anggraeni Janar Wulan Universitas Lampung

DOI:

https://doi.org/10.53089/medula.v13i5.608

Keywords:

CA1 layer, hippocampus, MSG, pyramidal cell, vitamin C

Abstract

Excessive consumption of MSG leads to decrease of the pyramidal cell of the CA1 layer of the hippocampus. Toxic level of glutamate causes overstimulation of glutamate receptor which leads to pyramidal cell death, thus results in decrease of pyramidal cell in CA1 layer of the hippocampus. Vitamin C as a strong antioxidant is expected to protect the oxidative damage in hippocampus caused by MSG induction. The aim of this research is to know the effect of vitamin C in pyramidal cell count of the CA1 layer of the hippocampus on male albino rat induced by MSG. This study is an experimental with Post Test Only Control Group contains of 30 rats divided into 5 groups which are, negative control (given aquadest), positive control (given MSG 4 g/kgBB), treatment 1 (given MSG 4 g/kgBB and vitamin C 100 mg/kgBB), treatment 2 (given MSG 4 g/kgBB and vitamin C 200 mg/kgBB) and treatment 3 (given MSG 4 g/kgBB and vitamin C 400 mg/kgBB) for 21 days. The measurement was done by using imageJ program in five microscopic field of five slices of the hippocampal CA1 layer. The analysis of vitamin C in pyramidal cell count of CA1 layer of hippocampus shows that there is significant difference (p=0,00). Vitamin C alter the decrease of CA1 hippocampal pyramidal cell of male albino rat induced by MSG.

References

Edward Z. Pengaruh pemberian Monosodium Glutamat (MSG) pada tikus jantan (Rattus norvegicus) terhadap FSH dan LH. M ked And. 2010; 34(2): 160-6.

Bera TK, Kar SK, Yadav PK, Mukherjee P, Yadav S, Joshi B. Effects of Monosodium Glutamate on human health: A systematic review. World J Pharm Sci. 2017; 5(5): 139-44.

Nuryani H, Jinap S. Soy sauce and its umami taste: A link from the past to current situation. J Food Sci. 2010; 5(3): 71-76.

Kazmi Z, Fatima I, Perveen S, Malik SS. Monosodium Glutamate: review on clinical reports. 2017; 20(52): 1807-15.

Collison, KS, Makhoul NJ, Zaidi MZ, AlRabiah R., Inglis A, Andres BL, Ubungen, R, Shoukri M, Al-Mohanna FA. Interactive effects of neonatal exposure to Monosodium Glutamate and aspartame on glucose homeostasis. Nutr & Met. 2012; 9(1): 58.

Snell RS. Snell’s clinical anatomy by regions. 10th Edition. Philadelphia: Lippincott Williams & Wilkins; 2018.

Liu J, Wang A, Huang Y, Xue P, Hao A. Oxidative stress mediates hippocampal neuron death in rats after lithium-pilocarpine-induced status epilepticus. J Seiz. 2010; 19(3): 165-72.

Muir GM, Bilkey DK. Instability in the place field location of hippocampal place cells after lesions centered on the perirhinal cortex. J Neurosc. 2001; 21(11): 4016-25.

Butler TR, Self RL, Smith KJ, Sharrett-Field LJ, Berry JN, Littleton JM, Pauly JR, Mulholland PJ, Prendergast MA. Selective vulnerability of hippocampal cornu ammonis 1 pyramidal cells to excitotoxic insult is associated with the expression of polyamine-sensitive N-methyl-D-asparate-type glutamate receptors. J Neurosc. 2010; 165(2): 525-34.

Monno AVA, Salmona M, Garattini S. Extracellular glutamate levels in the hypothalamus and hippocampus of rats after acute or chronic oral intake of monosodium glutamate. Neurosci Let. 1995; 193: 45-48.

Un I, Buyukuslu N. Maternal monosodium glutamate intake influences the learning ability of the offspring of sprague dawley rats. Acta Pharm Sci. 2018; 56(4): 107-18.

Moneim WMA, Yassa HA, Makboul RA, Mohamed NA. Monosodium glutamate affects cognitive functions in male albino rats. Eg J For Sci. 2018; 8(9): 1-10.

Dayono B, Trianto HF, Ilmiawan MI. Histologi sel piramidal hippocampus tikus putih pasca penghentian pajanan Monosodium Glutamat peroral. J Vok Kes. 2015; 1(4): 124-30.

Hansen SN, Nyborg PT, Lykkesfeldt J. Does vitamin C deficiency affect cognitive development and function. J Nutr. 2014; 6: 3818-46.

Ishaq GM, Saidu Y, Bilbis LS, Muhammad SA, Jinjir N, Shehu BB. J Neurosci rural pract. 2013; 4(3): 292-7.

Bisong SA, Ajiwhen IO, Mfem CC, Igiri AO. Effect of Vitamin C Supplementation on Learning and Memory in CD1 Mice. Brit J Med & Res. 2016; 16(10): 1-10.

Farombi OA, Onyema EO. Monosodium Glutamate-induced oxidative damage and genotoxicity in the rat: modulatory rote of vitamin C, vitamin E and quercetin. Hum & Exp Tox. 2006; 26: 251-9.

Shah SA, Yoon GH, Kim HO, Kom MO. Vitamin C neuroprotection against dose-dependent glutamat-induced neurodegneration in the postnatal brain. Neurochem Res. 2015.

Suzan GR, Pietro MG, Ana PP, Cristina WN. Monosodium Glutamate induced nociception and oxidative stress dependent on time of administration, age of rats and susceptibility of spinal cord and brain. J Taap. 2017.

Veronika H, Daniela O. Monosodium glutamate toxic effects and their implications for human intake: A review. JMED Res. 2013; 1-12.

Narayanan SN, Kumar RS, Paval J, Nayak S. Effect of ascorbic acid on the monosodium glutamate-induced neurobehavioral changes in periadolescent rats. Bratisl Lek Listy. 2010; 115(5): 247-52.

Afifi OK, Embaby AS. Histological study on the protective role of ascorbic acid on cadmium induced cerebral cortical neurotoxicity in adult male albino rats. J Micros & Ultras. 2015; 4(1): 36-45.

Pinto AC, Acuña AI, Beltrán FA, Díaz LT, Castro MA. 2015. Old things new view: Ascorbic acid protects the brain in neurodegenerative disorders. J Mol Sci. 16: 28194–217.

Sepehri H, Ganji F. The protective role of ascorbic acid on hippocampal CA1 pyramidal neurons in a rat model of maternal lead exposure. J Chem Neur. 2016; 74: 5-10.

Ezza HSA, Khadrawyb YA. Glutamate excitotoxicity and neurodegeneration. J Mol Gen Med. 2014; 8: 4.

Shahidi S, Komaki A, Mahmoodi M, Atrvash N, Marzieh G. Ascorbic acid supplementation could affect passive avoidance learning and memory in rat. B Res Bull. 2008; 76(1-2): 109-113.

Moretti M, Budni J, Dos-Santos DB, Antunes A, Daufenbach JF, Manosso LM, Farina M, Rodrigues AL. Protective effects of ascorbic acid on behavior and oxidative status of restraint-stressed mice. J Mol Neurosi. 2013; 49(1): 68-79.

Clemens Z, Toth C. Vitamin C and disease: Insights from the evolutionary perspective. J Evo & Hea. 2016; 1(1): 1-22.

Simon H, Muhartomo H, Pudjonarko D. Pengaruh pemberian monosodium glutamat peroral terhadap degenerasi neuron piramidal CA1 hipokampus pada tikus wistar. Med Hosp. 2013; 1(3): 175-81.

Paradiso B, Simonanto M, Thiene G, Lavezzi A. From fix to fit into the autoptic human brains. Eur J Histochem. 2018; 62: 2294-303.

Arjadi F, Soejono SK, Maurits LS, Pangestu M. Jumlah sel piramidal CA3 hipokampus tikus putih jantan pada berbagai model stres kerja kronik. M Ked Band. 2014; 46(4): 197-202.

Barr JL, Bray B, Forster GL. The hippocampus as a neural link between negative affect and vulnerability for psychostimulant relapse. Intech Op. 2018; 127-67.

Published

2023-07-15

How to Cite

Shinta Melia Desiana, Susianti, S., & Wulan, A. J. (2023). The Effect of Vitamin C in Pyramidal Cell Count of CA1 Layer of The Hippocampus on Male Albino Rat (Rattus norvegicus) Sprague Dawley Strain Induced by Monosodium Glutamate (MSG). Medical Profession Journal of Lampung, 13(5), 816-826. https://doi.org/10.53089/medula.v13i5.608

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